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J Vet Sci 2018; 19(6): 808-816  https://doi.org/10.4142/jvs.2018.19.6.808
Combination of berberine and ciprofloxacin reduces multi-resistant Salmonella strain biofilm formation by depressing mRNA expressions of luxS, rpoE, and ompR
Chenxi Shi1,2,†, Minmin Li1,2,†, Ishfaq Muhammad1,2, Xin Ma3, Yicong Chang1,2, Rui Li1,2, Changwen Li3, Jingshan He1,2, Fangping Liu1,2,*
1College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
2Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin 150030, China
3Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150069, China
Correspondence to: Tel: +86-451-55190674; Fax: +86-451-55191200; E-mail: fangpingliu@126.com
The first two authors contributed equally to this work.
Received: July 12, 2018; Revised: September 13, 2018; Accepted: October 1, 2018; Published online: November 30, 2018.
Abstract
Bacterial biofilms have been demonstrated to be closely related to clinical infections and contribute to drug resistance. Berberine, which is the main component of Coptis chinensis, has been reported to have efficient antibacterial activity. This study aimed to investigate the potential effect of a combination of berberine with ciprofloxacin (CIP) to inhibit Salmonella biofilm formation and its effect on expressions of related genes (rpoE, luxS, and ompR). The fractional inhibitory concentration (FIC) index of the combination of berberine with CIP is 0.75 showing a synergistic antibacterial effect. The biofilm’s adhesion rate and growth curve showed that the multi-resistant Salmonella strain had the potential to form a biofilm relative to that of strain CVCC528, and the antibiofilm effects were in a dose-dependent manner. Biofilm microstructures were rarely observed at 1/2 × MIC/FIC concentrations (MIC, minimal inhibition concentration), and the combination had a stronger antibiofilm effect than each of the antimicrobial agents used alone at 1/4 × FIC concentration. LuxS, rpoE, and ompR mRNA expressions were significantly repressed (p < 0.01) at 1/2 × MIC/FIC concentrations, and the berberine and CIP combination repressed mRNA expressions more strongly at the 1/4 × FIC concentration. The results indicate that the combination of berberine and CIP has a synergistic effect and is effective in inhibiting Salmonella biofilm formation via repression of luxS, rpoE, and ompR mRNA expressions.
Keywords: Salmonella, berberine, biofilm, drug combinations, multidrug resistance


© 2018 The Korean Society of Veterinary Science.

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