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J Vet Sci 2018; 19(6): 817-826  https://doi.org/10.4142/jvs.2018.19.6.817
Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine
Cong Zhang, Jiangfei Zhou, Zhixin Liu, Yongqing Liu, Kairui Cai, Tengfei Shen, Chengshui Liao, Chen Wang*
Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology, Luoyang 471023, China
Correspondence to: Tel: +86-13693839532; Fax: +86-379-64282341; E-mail: wangchen2001@163.com
The first two authors contributed equally to this work.
Received: May 28, 2018; Revised: July 22, 2018; Accepted: August 15, 2018; Published online: November 30, 2018.
Abstract
The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.
Keywords: adjuvants, bursal peptides-(I–IV), cellular immune response, humoral immune response, immune protection


© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.