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Expression of polo-like kinase 1 in pre-implantation-stage murine somatic cell nuclear transfer embryos
Jeonghyeon Moon, Sangho Roh*
Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute, BK21, Seoul National University School of Dentistry, Seoul 08826, Korea
Correspondence to: Tel: +82-2-880-2333; E-mail: sangho@snu.ac.kr
Received: May 15, 2018; Revised: October 23, 2018; Accepted: November 6, 2018; Published online: November 26, 2018.
Somatic cell nuclear transfer (SCNT) has various abilities of application in research, as well as the medical field and industry. However, the efficiency of SCNT is very low because the accurate mechanism of SCNT murine embryo development is still unknown. In previous studies, Polo-like kinase 1 (Plk1) has been found to be a crucial element in cell division including centrosome maturation, cytokinesis and spindle formation, and, in general, the developmental rate of SCNT murine embryos is lower than in vivo counterpart. In the first series of the experiments, BI2536, a Plk1 inhibitor, was treated to in vivo-fertilized embryos and the embryos failed to develop over the 2-cell stage. This re-confirms that Plk1 is crucial for the first mitotic division of murine embryos as previously reported by others. Next, we checked the Plk1’s localization and intensity by immunofluorescence. The SCNT murine embryos, which were not developed, have two types of different Plk1 expressions in comparison with normal developed embryos. One is a, low expression pattern of Plk1 and the other is ectopic expression of Plk1. Therefore, this result shows that Plk1 also plays critical role in SCNT murine embryos. In conclusion, this study demonstrated that the SCNT murine embryos which failed to develop over 2-cell stage show abnormal expression patterns of Plk1, and this may one of the main causes of developmental failure of early SCNT murine embryos.
Keywords: Polo-like kinase 1 (Plk1), Somatic cell nuclear transfer (SCNT), Development, mitotic division, mouse embryo

© 2018 The Korean Society of Veterinary Science.