• home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
Dimas Arya Abdillah, Setyawan EMN, Min Jung Kim, Hyun Ju Oh, Seok Hee Lee, Kihae Ra, Byeong Chun Lee*
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
Correspondence to: Tel: +82-2-880-1269; Fax: +82-2-873-1269; E-mail: bclee@snu.ac.kr
Received: September 10, 2018; Revised: November 8, 2018; Accepted: November 18, 2018; Published online: November 26, 2018.
Abstract
The objectives of this study were to analyze the protective effects of OptiprepTM (iodixanol) on dog spermatozoa during cryopreservation. The optimal concentration of OptiprepTM, 2.5%, was determined using fresh spermatozoa, and applied for the following experiment. The 2.5% OptiprepTM group showed significantly increased sperm motility than the control (P < 0.05). Lower mitochondrial ROS modulator (ROMO1) and pro-apoptotic gene (BAX), together with higher expression of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic gene (BCL2) and sperm acrosome associated-3 (SPACA3), were expressed in the iodixanol treated group. In addition, decreased protamine deficiency and cryocapacitation was observed in the treatment group. Our findings show that supplementation of 2.5% OptiprepTM is ideal for reducing production of ROS and preventing detrimental effects during cryopreservation process of canine sperm, manifested as increased motility and reduced cryocapacitation in the frozen-thawed spermatozoa.
Keywords: dog; cryocapacitation; cryopreservation; reactive oxygen species; protamine


© 2018 The Korean Society of Veterinary Science.