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Genetic identification of astroviruses in wild boars
Stefan Vilcek*, Slavomira Salamunova, Anna Jackova
University of Veterinary Medicine and Pharmacy, Komensky 73, SK-041 81 Kosice Slovakia
Correspondence to: Tel: +421-915-984654, E-mail: vilcek@uvm.sk
Received: October 10, 2018; Revised: November 8, 2018; Accepted: November 18, 2018; Published online: November 26, 2018.
Astroviruses were widely detected in pigs but their detection in wild boars is rather sporadic. In this study astroviruses were detected in organ homogenates of wild boars by a nested RT-PCR and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analysed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), and 2 as lineage 4 (PAstV-4). Two identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to bat astrovirus. The first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
Keywords: wild boar, porcine astrovirus, chicken astrovirus, bat astrovirus, phylogenetic analysis

© 2018 The Korean Society of Veterinary Science.