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J. Vet. Sci. 2019; 20(1): 16-26  https://doi.org/10.4142/jvs.2019.20.1.16
Correlation of spontaneous adipocyte generation with osteogenic differentiation of porcine skin-derived stem cells
Hyo-Kyung Bae1, Bae-Dong Jung1, Seunghyung Lee2, Choon-Keun Park2, Boo-Keun Yang2, Hee-Tae Cheong1,*
Colleges of 1Veterinaryy Medicine and Institute of Veterinary Science and 2Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea
Correspondence to: *Tel: +82-33-250-8659; Fax: +82-33-259-5625; E-mail: htcheong@kangwon.ac.kr
Received: September 18, 2018; Revised: October 18, 2018; Accepted: November 6, 2018; Published online: January 31, 2019.
The objective of this study was to examine effects of spontaneous adipocyte generation on osteogenic differentiation of porcine skin-derived stem cells (pSSCs). Correlation between osteogenic differentiation and adipocyte differentiation induced by osteocyte induction culture was determined using different cell lines. Osteogenic differentiation efficiency of pSSCs was then analyzed by controlling the expression of adipocyte-specific transcription factors during osteogenic induction culture. Among four cell lines, pSSCs-II had the lowest lipid droplet level but the highest calcium content (p < 0.05). It also expressed significantly low levels of peroxisome proliferator-activated receptor gamma 2 (PPARγ2) and adipocyte protein 2 (aP2) mRNAs but very high levels of runt-related transcription factor 2 (Runx2) and alkaline phosphatase (ALP) mRNAs as osteogenic makers (p < 0.05). Oil red O extraction was increased by 0.1 µM troglitazone (TGZ) treatment but decreased by 50 µM bisphenol A diglycidyl ether (BADGE) (p < 0.05). Calcium content was drastically increased after BADGE treatment compared to that in osteogenic induction control and TGZ-treated pSSCs (p < 0.05). Relative expression levels of PPARγ2 and aP2 mRNAs were increased by TGZ but decreased by BADGE. Expression levels of Rucx2 and ALP mRNAs, osteoblast-specific marker genes, were significantly increased by BADGE treatment (p < 0.05). The expression level of BCL2 like 1 was significantly higher in BADGE-treated pSSCs than that in TGZ-treated ones (p < 0.05). The results demonstrate that spontaneous adipocyte generation does not adversely affect osteogenic differentiation. However, reducing spontaneous adipocyte generation by inhibiting PPARγ2 mRNA expression can enhance in vitro osteogenic differentiation of pSSCs.
Keywords: Porcine skin-derived stem cells, Osteogenesis, Spontaneous adipocyte generation, Peroxisome proliferator-activated receptor gamma 2

© 2019 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.