Effects of irradiation and leukoreduction on down-regulation of CXCL-8 and storage lesion in stored canine whole blood

Hayoung Yang; Woosun Kim; Junwoo Bae; Hyunwoo Kim; Sangki Kim; Jihye Choi; Jinho Park; Dong-In Jung; HongBum Koh; DoHyeon Yu

doi:10.4142/jvs.2019.20.1.72

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J. Vet. Sci. 2019; 20(1): 72-78 https://doi.org/10.4142/jvs.2019.20.1.72

Effects of irradiation and leukoreduction on down-regulation of CXCL-8 and storage lesion in stored canine whole blood

Hayoung Yang¹, Woosun Kim¹, Junwoo Bae¹, Hyunwoo Kim¹, Sangki Kim², Jihye Choi¹, Jinho Park³, Dong-In Jung⁴, HongBum Koh^1,*, DoHyeon Yu^4,*

¹College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea
²College of Industrial Science, Kongju National University, Yesan 32439, Korea
³College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea
⁴Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Korea

Correspondence to: ^*Tel: +82-55-772-2368; Fax: +82-55-772-2330; E-mails: yudh@gnu.ac.kr(DH Yu), hbkoh@chonnam.ac.kr (HB Koh)

Received: August 1, 2018; Revised: November 27, 2018; Accepted: November 30, 2018; Published online: January 31, 2019.

Abstract: White blood cells (WBCs) and storage period are the main factors of transfusion reactions. In the present study, cytokine/chemokine concentrations after leukoreduction (LR) and irradiation (IR) in stored canine whole blood were measured. Red blood cell storage lesion caused by IR and LR were also compared. Blood samples from 10 healthy Beagles were divided into four groups (no treatment, LR-, IR-, and LR + IR-treated). Leukocytes were removed by filtration in the LR group and gamma radiation (25 Gy) was applied in the IR group. Immunologic factors (WBCs, interleukin-6 [IL-6], C-X-C motif chemokine ligand 8 [CXCL-8], and tumor necrosis factor-alpha) and storage lesion factors (blood pH, potassium, and hemolysis) were evaluated on storage days 0, 7, 14, 21, and 28. Compared to the treated groups, IL-6 and CXCL-8 concentrations during storage were significantly higher in the control (no treatment) group. LR did not show changes in cytokine/chemokine concentrations, and storage lesion presence was relatively mild. IR significantly increased CXCL-8 after 14 days of storage, but IR of leukoreduced blood did not increase CXCL-8 during 28 days of storage. Storage lesions such as hemolysis, increased potassium, and low pH were observed 7 days after IR and storage of blood, regardless of LR. IR of leukoreduced blood is beneficial to avoid immune reactions; however, storage lesions should be considered upon storage.; Keywords: Blood preservation, Irradiation, Leukocyte reduction procedures, Transfusion, Cytokine

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