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J. Vet. Sci. 2019; 20(1): 79-86  https://doi.org/10.4142/jvs.2019.20.1.79
Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm
Dimas A. Abdillah, Erif M. N. Setyawan, Hyun Ju Oh, Kihae Ra, Seok Hee Lee, Min Jung Kim, Byeong Chun Lee*
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
Correspondence to: *Tel: +82-2-880-1269; Fax: +82-2-873-1269; E-mail: bclee@snu.ac.kr
Received: September 10, 2018; Revised: November 8, 2018; Accepted: November 18, 2018; Published online: January 31, 2019.
Abstract
The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control (p < 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator (ROMO1) and pro-apoptotic gene (BAX) expressions, together with higher expressions of protamine-2 (PRM2), protamine-3 (PRM3), anti-apoptotic B-cell lymphoma-2 (BCL2), and sperm acrosome associated-3 (SPACA3) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa.
Keywords: Dogs, Sperm, Cryopreservation, Reactive oxygen species, Protamine


© 2019 The Korean Society of Veterinary Science.

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