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J. Vet. Sci. 2019; 20(1): 91-94  https://doi.org/10.4142/jvs.2019.20.1.91
Genetic identification of astroviruses in wild boars
Stefan Vilcek*, Slavomira Salamunova, Anna Jackova
Department of Epizootiology and Parasitology, University of Veterinary Medicine and Pharmacy, SK-041 81 Košice, Slovakia
Correspondence to: *Tel: +421-915-984654; E-mail: vilcek@uvm.sk
Received: October 10, 2018; Revised: November 8, 2018; Accepted: November 18, 2018; Published online: January 31, 2019.
Astroviruses are widely detected in pigs but their detection in wild boars is rather sporadic. In this study, astroviruses were detected in organ homogenates of wild boars by applying nested reverse transcriptase polymerase chain reaction, and the typing was carried out by phylogenetic analysis. Overall, 30/200 (15.0%) homogenates were positive for astroviruses. Genetic typing revealed that of 13 amplicons analyzed, 8 were typed as porcine astrovirus lineage 2 (PAstV-2), 2 as lineage 4 (PAstV-4), 2 identical sequences were grouped with chicken astrovirus, and 1 sequence belonged to a bat astrovirus lineage. This first identification of chicken and bat astroviruses in wild boars indicates interspecies transmission.
Keywords: Wild boar; Porcine astrovirus, Chicken astrovirus, Bat astrovirus, Phylogenetic analysis

© 2019 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.