• home
  • home
  • articles
  • authors
  • Reviewers
  • About the Journal
  • About the Journal
  • About the Journal
  • About the Journal
  • e-Submission

Indexed/Covered by

J. Vet. Sci. 2010; 11(1): 43-50  https://doi.org/10.4142/jvs.2010.11.1.43
Ethanol extract of Angelica gigas inhibits croton oil-induced
inflammation by suppressing the cyclooxygenase-prostaglandin
pathway
Sunhee Shin1, Seong Soo Joo1, Dongsun Park1, Jeong Hee Jeon1, Tae Kyun Kim1, Jeong Seon Kim3, Sung Kyeong
Park3, Bang Yeon Hwang2,*, Yun-Bae Kim1,*
1College of Veterinary Medicine and Research Institute of Veterinary Medicine, and 2College of Pharmacy, Chungbuk
National University, Cheongju 361-763, Korea
3Daejeon Health Sciences College, Daejeon 300-711, Korea
Abstract
The anti-inflammatory effects of an ethanol extract of Angelica
gigas (EAG) were investigated in vitro and in vivo using
croton oil-induced inflammation models. Croton oil (20
μg/mL) up-regulated mRNA expression of cyclooxygenase
(COX)-I and COX-II in the macrophage cell line, RAW
264.7, resulting in the release of high concentrations of
prostaglandin E2 (PGE2). EAG (1∼10 μg/mL) markedly
suppressed croton oil-induced COX-II mRNA expression
and PGE2 production. Application of croton oil (5% in
acetone) to mouse ears caused severe local erythema, edema
and vascular leakage, which were significantly attenuated
by oral pre-treatment with EAG (50∼500 mg/kg). Croton
oil dramatically increased blood levels of interleukin (IL)-6
and PGE2 without affecting tumor-necrosis factor (TNF)-α
and nitric oxide (NO) levels. EAG pre-treatment remarkably
lowered IL-6 and PGE2, but did not alter TNF-α or NO
concentrations. These results indicate that EAG attenuates
inflammatory responses in part by blocking the COX ????
PGE2 pathway. Therefore, EAG could be a promising
candidate for the treatment of inflammatory diseases.
Keywords: Angelica gigas, croton oil, cyclooxygenase-II,
inflammation, prostaglandin E2
© 2010 The Korean Society of Veterinary Science.