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J Vet Sci 2018; 19(1): 13-20  https://doi.org/10.4142/jvs.2018.19.1.13
Comparison of the characteristics of canine adipose tissue-derived mesenchymal stem cells extracted from different sites and at different passage numbers
Kevin M. Yaneselli1, Cristiana P. Kuhl2, Paula B. Terraciano2, Fernanda S. de Oliveira2, Sabrina B. Pizzato2, Kamila Pazza2, Alessandra B. Magrisso2, Vanessa Torman3, Analía Rial4, María Moreno4, Silvia Llambí5, Elizabeth Cirne-Lima2, Jacqueline Maisonnave1,*
1Laboratory of Immunology, Department of Microbiological Science, Faculty of Veterinary, 4Laboratory for Vaccine Research, Department of Biotechnology, Instituto de Higiene, Faculty of Medicine, and 5Laboratory of Genetics, Faculty of Veterinary, Universidad de la República, Montevideo 11600, Uruguay
2Laboratory of Embryology and Cellular Differentiation, and 3Biostatistics, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS 90035-903, Brazil
Correspondence to: Jacqueline Maisonnave
Tel: +598-26281303; Fax: +598-26280130; E-mail: jacmaiso@gmail.com
Received: December 28, 2016; Revised: April 25, 2017; Accepted: May 5, 2017; Published online: January 31, 2018.
Abstract
Mesenchymal stem cells (MSCs) have desirable characteristics for use in therapy in animal models and veterinary medicine, due to their capacity of inducing tissue regeneration and immunomodulation. The objective of this study was to evaluate the differences between canine adipose tissue-derived MSCs (AD-MSCs) extracted from subcutaneous (Sc) and visceral (Vs) sites. Surface antigenic markers, in vitro differentiation, and mineralized matrix quantification of AD-MSCs at different passages (P4, P6, and P8) were studied. Immunophenotypic analysis showed that AD-MSCs from both sites were CD44+, CD90+, and CD45-. Moreover, they were able, in vitro, to differentiate into fat, cartilage, and bone. Sc-AD-MSCs preserve in vitro multipotentiality up to P8, but Vs-AD-MSCs only tri-differentiated up to P4. In addition, compared to Vs-AD-MSCs, Sc-AD-MSCs had greater capacity for in vitro mineralized matrix synthesis. In conclusion, Sc-AD-MSCs have advantages over Vs-AD-MSCs, as Sc AD-MSCs preserve multipotentiality during a greater number of passages, have more osteogenic potential, and require less invasive extraction.
Keywords: canine, immunophenotyping, mesenchymal stem cells, multipotent plasticity
© 2018 The Korean Society of Veterinary Science.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.